Molecular Pathology

An developing field of pathology called molecular pathology is concerned with studying and diagnosing disease by looking at molecules in organs, tissues, or physiological fluids. Molecular pathology, which is frequently referred to as a “crossover” field, shares some features of practise with both anatomic pathology and clinical pathology, molecular biology, biochemistry, proteomics, and genetics It is multidisciplinary in nature and primarily focuses on the microscopic characteristics of disease. One important factor to keep in mind is that when the diagnosis is based on both the morphologic alterations in tissues and on molecular testing, a more accurate diagnosis may be feasible.

Molecular Testing For Diagnosis

Early methods of molecular diagnosis tended to concentrate on mutations that were directly linked to a given illness or condition. 20, 21, 22 These diagnostic tests have played a significant role in helping pathologists create sophisticated categorization schemes and make more accurate diagnoses. The challenges of acquiring enough fresh tissue for current methods hindered early testing.

What Are Molecular Pathology Techniques?

Molecular profiling of pathogens, multiplex PCR, DNA microarray, in situ hybridization, in situ RNA sequencing, DNA sequencing, antibody-based immunofluorescence tissue tests, and examination of bacterial genes for antibiotic resistance are all examples of quantitative polymerase chain reactions.

What Are Molecular Diagnostic Techniques?

The investigation of human, viral, and microbial genomes, genes, and the products they encode in clinical and public health laboratories has been transformed by molecular diagnostic testing, which combines laboratory testing with the accuracy of molecular biology.

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